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Interests and Research Activities
Dr. Sabbadini has been interested in the role of
sphingolipid second messengers in disease, including
cancer inflammation and heart disease. Animal and
human studies of heart disease and cancer are explored.
Dr. Sabbadini's lab is focused on developing novel
therapeutics for the treatment of sphingolipid-related
diseases. Sphingolipids are structural and multifunctional
lipid mediators largely involved in signaling activities
required for normal cellular function. However,
sphingolipids can become dysfunctional and directly
contribute to the pathophysiology of cancer, inflammation,
angiogenesis and cardiovascular diseases. Sabbadini
is developing assays, inhibitors, and activators
in order to identify and develop therapeutics that
act upon sphingolipids involved in disease pathways.
REPRESENTATIVE PUBLICATIONS
Krown, K., K. Yasui, M. Brooker, A. Dubin, C. Nguyen,
G. Harris, P. McDonough, C. Glembotski, P. Palade
and R. Sabbadini. TNFa receptor expression in rat
cardiac myocytes: TNFa inhibition of L-type Ca2+
current and Ca2+ transients. FEBS Letters 376:24-30.
1995.
Krown, K., M. T. Page, C. Nguyen, V. Gutierrez,
K. L. Comstock, P. J. E. Quintana, C. C. Glembotski
& R. A. Sabbadini. TNFa-induced apoptosis in
cardiac myocytes: Involvement of the sphingolipid
signalling cascade in cardiac cell death. J. Clin.
Invest. 98:2854-2865. 1996.
Betto, R., A. Teresi, F. Turcato, G. Salviati, R.
Sabbadini, K. Krown, C. Glembotski, C. Dettbarn,
K. Yasui, P. Palade. Sphingosylphosphorylcholine
modulates the ryanodine receptor calcium release
channel of cardiac sarcoplasmic reticulum membranes.
Biochem. J. 322:327-333. 1997.
Zechner, D., R. Craig, D. Hanford, P.M. McDonough,
R. A. Sabbadini and C.C. Glembotski. MKK6 inhibits
myocardial cell apoptosis via a p38 MAP kinase-dependent
mechanism. J. Biol. Chem. 272:8232-8239. 1998.
Kevyn L. Comstock, Kevin A. Krown, M. Trevor Page,
Don Martin, Peter Ho, Melinda Pedraza, Ernesto N.
Castro, Nobuko Nakajima, Christopher C. Glembotski,
P.J. Quintana and Roger Sabbadini. LPS-induced TNFa
release from and apoptosis in rat cardiomyocytes:
Obligatory role of CD14 in mediating the LPS response.
J. Mol. Cell. Cardiol. 30:2761-2775. 1998.
Ghosh, N. R. Sabbadini and S. Chatterjee. Identification,
partial purification and localization of a neutral
sphingomyelinase in rabbit skeletal muscle: neutral
sphingomyelinase in skeletal muscle. Mol. Cell.
Biochem. 189: 161-168. 1998.
Krown, K. and R. A. Sabbadini. Preparation and Use
of Myocardial Cells in Culture to Study Effects
of Cytokines in Methods in Molecular Medicine: Septic
Shock Methods and Protocols.Humana Press. Ed. T.
Evans. 36:181-188. 1999.
Sabbadini, R.A., D. Danielli-Betto and R. Betto.
Role of sphingolipids in muscle function. Ital.
Journal of Neurological Sciences. 20:423-430. 1999.
Page, M.T., P.J.E. Quintana, J. A. Ligutti and R.
A. Sabbadini. Cell death in cultured adult rat cardiomyocytes:
use of the COMET assay to distinguish apoptosis
from necrosis. Basic and Appl. Myology 10: 159-170.
2000.
Nakajima, N.J. A. Ligutti, A. L. Cavalli, D. Biral,
C.C. Glembotski, P. M. McDonough, P. D. Ho, R. Betto,
D. Sandona, P. T. Palade, C. A. Dettbarn, R. E.
Klepper and R. A. Sabbadini. Expression and characterization
of Edg-1 receptors in rat cardiomyocytes: Calcium
deregulation in response to sphingosine-1-phosphate.
Eur. J. Biochem. 267: 5679-5686. 2000.
Ligutti, J., Nakajima, N., Cavalli, A.L, Betto,
R., Biral, D., Sabbadini, R. G protein-coupled receptors,
calcium deregulation and apoptosis in the heart.
Basic Appl. Myol. 10: 75-76. 2000..
Dalla Libera, L., R. Sabbadini, B. Ravara, M. Sandri,
R. Betto and G. Vescovo. Apoptosis in the skeletal
muscle of rats with heart failure is associated
with increase serum levels of TNFa and sphingosine.
J. Mol. Cell. Cardiol. 31: 1871-1878. 2001.
Betto, R. A. Teresi, F. Turcato, M. Duca, D. Danieli-Betto
and R. Sabbadini. Inability of sphingosine and calmodulin
to control ryanodine receptor in malignant hyperthermia.
Basic and Appl. Myology 12: 167-176. 2002.
Cavalli, A.L. J. A. Ligutti, N.M. Gellings, E. Castro,
M. T. Page, R. E. Klepper, and R. A. Sabbadini.
The role of TNFa and sphingolipid signaling in cardiac
hypoxia: Evidence that cardiomyocytes release TNFa
and sphingosine. Basic and Appl. Myology 12: 159-167.
2002.
Cavalli, A., N. O’Brien, S. Barlow, R. Betto,
C. Glembotski, P. Palade and R. Sabbadini. Expression
and functional characterization of SCaMPER: A calcium
channel of cardiomyocytes. Am. J. Physiol. 284:
C780-C790. 2003.
O’Brien, N, N. Gellings, M. Guo, C. C. Glembotski,
and R. A. Sabbadini. Molecular Identification of
Rat FAN and its Role in hypoxia reoxygenation induced
cell death in cardiomyocytes. Circ. Res., 92: 589-91.
2003.
Deutschman, D., Carstens, J.S., R. L. Klepper, W.
S. Smith, M. T. Page, T. R. Young, L. Gleason, N.
Nakajima and R. A. Sabbadini. Predicting coronary
artery disease using serum sphingosine-1-phosphate.
Am. Heart J. 146: 62-68. 2003.
Danieli-Betto, D., E. Germinario, A. Esposito, A.
Megeghian, M. Midrio, B. Ravara, E. Damiani, L.
Dalla Libera, R. A. Sabbadini and R. Betto. Sphingosine-1-phosphate
protects mouse EDL skeletal muscle during fatigue.
Am. J. Physiol. 288:C1367-73. 2005.
Ph.D. students: Nicole Gellings, Matt Giacalone
Contact information.
Dr. Roger Sabbadini
Department of Biology
SDSU Heart Institute
San Diego State University
San Diego, CA 92182-4614
(619) 594-6272 (office) 594-5623 (lab)
Fax (619) 594-0626
email: rsabba@sunstroke.sdsu.edu
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