The FACS Core at San Diego State University relies for now on the use of its BD FACSAria

 

The BD FACSAria is a powerful instrument that allows the precise analysis of the fluorescent properties of cells and the isolation of up to four different sub-populations at a time.

Instrumentation

 Blue Laser (488nm) Octagon:

Detector:    LP Mirror   BP Filter  Fluorochrome Example

A                   735        780/60          PE-Cy7

B                   655        675/20          PerCP, PerCP-Cy5.5, PI

C                   595        610/20          PE-Texas Red

D                   556        585/42          PI

E                   502        530/30          GFP

F                    -           488/10          SSC

Detector:    LP Mirror   BP Filter  Fluorochrome Ex:

Red (633nm) Laser Trigon:

A                  735         780/60        APC-Cy7

Use this info with BD’s Spectrum Viewer to optimize your experiment and to determine which detectors

 to use your fluorochrome with.

 

 

 

Our BD FACSAria has 3 lasers (405nm, 488nm, 633nm) with 11 different detectors in use.  Here is the current configuration:

 

 

 

 

 

B                  685         710/20         AlexaFluor700

C                                  660/20          APC

Detector:    LP Mirror   BP Filter  Fluorochrome Ex:

Violet (405nm) Laser Trigon:

A                  595           605/40           QDot605

B                   502          530/30           AmCyan

C                                   450/40           PacBlue

FACS Core Facility at SDSU