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The BD FACSAria flow cytometer is the first fixed-alignment benchtop highspeed cell sorter. The instrument can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD FACSAria flow cytometer enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at a time.  The FACSAria can sort up to four different cell populations simultaneously.

  1. Loads 5mL round bottom tubes or 15mL conical tubes

  2. Lasers: 405nm, 488nm, 633nm

  3. Detectors: 5 from blue laser, 3 from red, 3 from violet laser (See configuration)

  4. Sample Chilling Unit allows samples to be cooled both during acquisition as well as keeping collected samples cold.

  5. Sorts up to 4 populations into 5mL tubes or 2 populations into 15mL tubes.

  1. Loads individual 5mL tubes, a carousel of up to 40 5mL tubes, or plates.

  2. Lasers: 488nm, 633nm

  3. Detectors: 4 from blue, 2 from red laser. 

Designed for research use, the optional BD High Throughput Sampler (HTS) provides fully automated and rapid sample acquisition that can speed through a microtiter plate in less than 15 minutes, with less than 1% sample carryover. In high-throughput mode, fast acquisition speed is achieved by synchronizing two high-precision pumps for sample mixing, sample injection, and probe washing. Standard-throughput mode can be selected for acquisition of larger sample volumes. The HTS supports standard 96 and 384-well plates. It is operated using BD FACSDiva software, allowing users to define customized delivery protocols, mixing, wash, and analysis parameters.

BD FACSAria

Cell Sorter

BD FACSCanto

Cell Analyzer

High Throughput Sampler (HTS)

Automatic Cell Deposition Unit (ACDU)

Flow Cytometry Core Facility

San Diego State University

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